ABSTRACT
To determine the reference range of zinc in adult population [age range 20 to 29 years] of Lahore. It was a descriptive cross sectional study which was carried out from Jan- August 2012 in Chemical Pathology Department of University of Health Sciences, Lahore. Serum zinc concentration was measured by name atomic absorption spectrometry in randomly selected 450 healthy adults aged 20 to 29 years. After application of exclusion criteria reference values were determined in apparently healthy subjects according to guidelines of International Federation of Clinical Chemistry. The data was entered and analyzed using SPSS version 20.0. Serum Zn levels was expressed as Mean +/- SD. A total of 450 healthy subjects were included in this study. Out of these, 234 were females. Mean age was 25 +/- 0.13years. The mean concentration of zinc in serum of healthy individual was 24.02 +/- 7.03 pol/L [rangel 1.47-36.72]. The mean +/- SD for males subjects were 22.33 +/- 6.42 pmol/L[range11.93-32.4]. Similarly the mean +/- SD for females were 21.72 +/- 7.34 pmol/L [range9.94-36.87]. This study presents reference range for serum zinc concentration in adult population of Lahore. The results showed that there is significant difference in serum level of zinc among different countries. This study will help us in establishing reference ranges of trace elements on larger population in future
ABSTRACT
The objective of this study was to compare and correlate the analytical performance of D10 Hemoglobin Testing System based on Cation Exchange HPLC and Roche Hitachi 902 Immunoturbidimetric method. Subjects and Methods: A total of 110 patients of Type 2 Diabetes Mellitus were included in the study. HbA1c was determined using D-10 Hemoglobin Testing system and Roche Hitachi 902 Analyzer. Both methods showed good correlation with the correlation coefficient [r] of 0.95. Between run Coefficient of variance was found to be lower for HPLC system compared to immunoturbidimtric method. HPLC method also produces a chromatogram that shows the different hemoglobin fractions, allowing identification of different hemoglobin variants. In the present study both methods showed good correlation but the D10 HPLC system provided adequate throughput and improved precision as compared to immunoturbidimetric method